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"This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. This book is an essential laboratory manual for all undergraduates and graduates about to embark on a cell culture project. It is a book which both experienced researchers and those new to the field will find invaluable."@en
"This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. This book is an."@en
""Animal Cell Culture: Essential Methods is an up-to-date guide to all the core concepts and techniques central to the performance of cell culture in the modern laboratory. Every chapter provides selected tried-and-tested protocols for immediate use at the bench along with a plethora of good practical advice, whilst also giving the essential background and theory, and discussing the merits and limitations of the various approaches. It represents the most accessible introduction available to the culture and manipulation in vitro of animal cells. The book is an essential laboratory manual for anyone in academia or industry who is about to embark on cell culture work, yet it will be no less useful to the experienced worker as an easy reference guide to established approaches and techniques. Accessible and up-to-date guide to a broad range of methods in animal cell culture. Explains clearly and precisely how to carry out selected techniques in addition to giving background information on the various approaches. Chapters are written by leading international authorities in the field and cover both well-known and new, tried and tested, methods for working in animal cell culture. An essential laboratory manual for students, researchers, and industrial scientists. Covers cryopreservation, the design and use of serum- and protein-free media and systems for culture scale-up."--P. 4 de la couv."
"Abstract: Animal cell culture is a widely used technology for producing recombinant proteins. The ability to make post-translational modifications and secrete the active forms of the protein into the culture medium represents major advantages over other processes. The growing market demand for pharmaceuticals has created a need for increased production capacity; however, achieving productivity gains in both the upstream stage and downstream processes can subject cells to aggressive environments such as those involving hydrodynamic stresses. Although numerous studies have explored the consequences of cell damage due to hydrodynamic stress, there has been a lack of understanding of the mechanism of such damage at a cellular level. Cell damage can also influence biomedical applications. Cells manipulated in instruments such as diagnosis and analysis devices can experience hydrodynamic forces."@en
"Abstract: Animal cell culture is a widely used technology for producing recombinant proteins. The ability to make post-translational modifications and secrete the active forms of the protein into the culture medium represents major advantages over other processes. The growing market demand for pharmaceuticals has created a need for increased production capacity; however, achieving productivity gains in both the upstream stage and downstream processes can subject cells to aggressive environments such as those involving hydrodynamic stresses. Although numerous studies have explored the consequences of cell damage due to hydrodynamic stress, there has been a lack of understanding of the mechanism of such damage at a cellular level. Cell damage can also influence biomedical applications. Cells manipulated in instruments such as diagnosis and analysis devices can experience hydrodynamic forces."