Genome analysis is essential both to understanding the molecular bases of physiological processes and to the development of novel therapies for treating human diseases. In Genomics Protocols, an expert panel of internationally acclaimed researchers from academia, industry, and leading genome centers provides a comprehensive generic set of protocols for gene hunting and genome analysis. Drawing on emerging technologies in the fields of bioinformatics and proteomics, these protocols cover not only those traditionally recognized as genomics, but also early therapeutic approaches exploiting the potential of gene therapy. Highlights include methods for the analysis of differential gene expression, SNP detection, comparative genomic hybridization, and the functional analysis of genes, as well as the use of bioinformatics for gene identification and the prediction of protein function. Each method includes step-by-step instructions and invaluable notes that describe the quirks in a procedure and the little tricks that make all the difference to a successful outcome. Comprehensive and eminently practical, Genomics Protocols provides academic and pharmaceutical researchers alike with richly detailed accounts of the most up-to-date genomics techniques, including the emerging technologies from proteomics-all highly effective tools that will help investigators expand their research horizons and better understand gene function.
"A team of researchers share the most current information in a field that has recently switched emphasis from gene identification to functional genomics and the characterization of genes and gene products. This volume approaches its complex subject with a broad perspective to supply its reader with a vital overview of genomics and its derivative fields, with a focus on pivotal issues such as data analysis."
"Construction of microsatellite-based, high-resolution genetics maps in the mouse (paul A. Lyons). Genetic analysis of complex traits (Stephen P. Bryant, Mathias N. Chiano). Sequence-based detection of single nucleotide polymorphisms (Deborah A. Nickerson, Natali Kolker, Scott L. Taylor, Mark J. Rieder). Genomic mistmatch scanning for the mapping of genetic traits (Farideh Mirzayans, Michael A. Walter). Detection of chromosomal abnormalities by comparative genomic hybridization ( Mario A. J. A. Hermsen, Marjan M. Weiss, Gerrit A. meijer, Jan P. A. Baak). Construction of a bacterial artificial chromosome library (Sangdun Choi, Ung-Jin Kim). Contiguation of bacterial clones (Sean J. Humpray, Susan J. Knaggs, Ioannis Ragoussis). Mapping of genomic clones by fluorescence in situ hybridization (Margaret A. Leversha). Map integration: from a genetic map to a physical gene map and ultimately to the sequence map (Panagiotis Deloukas). Construction of full-lenght-enriched cDNA libraries: the Oligo-capping method (Yutaka Suzuki, Sumio Sugano). Construction of transcript maps by somatic cell/radiation hybrid mapping: the human gene map (Panagiotis Deloukas). Preparation and screening of high-density cDNA arrays with genomic clones (Gunther Zehetner, Maria Pack, Katja Schafer). Direct selection of cDNAs by genomic clones (Daniela Toniolo). Exon trapping: application of a large-insert multiple-exon-trapping system (Martin C. Wapenaar, Johan T. Den Dunnen). Sequencing bacterial artificial chromosomes (David E. Harris, Lee Murphy). Finding genes in genomic nucleotide sequences by using bioinformatics (Yvonne J. K. Edwards, Simon M. Brocklehurst). Gene identification using the pufferfish, fugu rubripes, by sequence scanning (Greg Elgar). Isolation of differentially expressed genes through subtractive suppression hybridization (Oliver Dorian von Stein). Isolation of differentially expressed genes by representational difference analysis (Christine Wallrapp, Thomas M. Gress). Expression profiling and the isolation of differenttially expressed genes by indexing-based differential display (Michael P. Starkey). Expression profiling by systematic high-throughput in situ hybridazition to whole-mount embryos (N. Pollet, C. Niehrs). Expression monitoring using cDNA microarrays: a general protocol (X. Jian Lou, M. Schena, F. T. Horrigan, R. M. Lawan, R. W. Davis). Prediction of protein structure and function by using bioinformatics (Y. J. K. Edwards, A. Cottage). Identification of novel genes by gene trap mutagenesis (A. K. Voss, T. Thomas). Determination of gene function by homologus recombination using embryonic stem cells and knockout mice (A. Mansori). Genomic analysis utilizing the yeast two-hybrid system (I. G. Serebriiskii, G. G. Toby, R. L. Finley Junior, E. A. Golemis). Methods for adeno-associated virus-mediated gene transfer into muscle (T. J. Amiss, R. J. Samulski). Retroviral-mediated gene transduction (D. S. Anson). Gene therapy approaches to sensitization of human prostate carcinoma to cisplatin by adenoviral expression of p53 and by antisense Jun kinase oligonucleotide methods (R. Gjerset, A. Haghighi, S. Lebedeva, D. Mercola). Ribozyme gene therapy (L. A. Phylactou). Index."
"Genome analysis is essential both to understanding the molecular bases of physiological processes and to the development of novel therapies for treating human diseases. In Genomics Protocols, an expert panel of internationally acclaimed researchers from academia, industry, and leading genome centers provides a comprehensive generic set of protocols for gene hunting and genome analysis. Drawing on emerging technologies in the fields of bioinformatics and proteomics, these protocols cover not only those traditionally recognized as genomics, but also early therapeutic approaches exploiting the potential of gene therapy. Highlights include methods for the analysis of differential gene expression, SNP detection, comparative genomic hybridization, and the functional analysis of genes, as well as the use of bioinformatics for gene identification and the prediction of protein function. Each method includes step-by-step instructions and invaluable notes that describe the quirks in a procedure and the little tricks that make all the difference to a successful outcome. Comprehensive and eminently practical, Genomics Protocols provides academic and pharmaceutical researchers alike with richly detailed accounts of the most up-to-date genomics techniques, including the emerging technologies from proteomics-all highly effective tools that will help investigators expand their research horizons and better understand gene function."@en
"Since the first edition, published in 2001, genomics research has taken great strides. In Genomics Protocols, 2nd Edition, a team of expert researchers share the most current information in a field that has recently switched emphasis from gene identification to functional genomics and the characterization of genes and gene products. This volume approaches its complex subject with a broad perspective to supply its reader with a vital overview of genomics and its derivative fields, with a focus on pivotal issues such as data analysis. Following the highly successful Methods in Molecular Biology series format, the chapters provide easy-to-follow laboratory protocols, lists of the necessary materials, and the excellent Notes section, which offers tips on troubleshooting and avoiding known pitfalls. Expansive and current, Genomics Protocols, 2nd Edition is the perfect update for researchers working in this integral, growing field."@en
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