"Human Genetics." . . "SCIENCE Biotechnology." . . "Polymerase Chain Reaction methods." . . "Proteòmica" . . "Life Sciences." . . "Mutagenitätstest." . . "Biomedicine." . . "Genètica humana" . . "Citologia" . . "Polimerasi Acido desossiribonucleico." . . "Induced mutation" . . "Ciències de la vida" . . "Vejledning" . . "Mutagenèse." . . "Mutagénèse." . "Mutagenese." . "PCR" . . "In vitro." . . "Biowissenschaften." . . "Manual" . . "Mutagènesi Metodologia." . . "Methods" . . "Mutagenesis, Insertional methods." . . "Induceret mutation, mutagenese" . . "Mutagenesis." . . "Protocole de recherche." . . "Méthodologie." . . "Labortechnik." . . "Genetics Methodology." . . "Mutageneza badania techniki." . . "Wetenschappelijke technieken." . . "Mutagenesis Methodology." . . "Proteomanalyse." . . "Mutagènesi." . . "Mutagenesis, Site-Directed." . . "Mutagenesi da inserzione metodi." . . "Polymerase chain reaction" . . "Medicina" . . "Aufsatzsammlung." . . "Electronic books." . . . . . "In Vitro Mutagenesis Protocols" . . "In the post-genome era, in vitro mutagenesis has emerged as the critically important tool used by molecular biologists in establishing the functions of components of the proteome. In this second edition of In Vitro Mutagenesis Protocols, active researchers with proven track records describe in stepwise fashion their advanced mutagenesis techniques. Each contributor focuses on improvements to conventional site-directed mutagenesis, with chapters being devoted to chemical site-directed mutagenesis; PCR-based mutagenesis and the modifications that allow high-throughput experiments; and mutagenesis based on gene disruption that is both in vitro- and in situ-based. Additional methods are provided for in vitro gene evolution; for gene disruption based on transposon, recombination, and cassette mutagenesis; and for facilitating the introduction of multiple mutations. Each readily reproducible technique includes detailed step-by-step instructions, tips on pitfalls to avoid, and notes on reagents and suppliers. Time-tested and highly practical, the techniques in In Vitro Mutagenesis Protocols, Second Edition offer today's molecular biologists a rich compendium of reliable and powerful techniques with which to illuminate the proteome."@en . . "Online-Publikation" . . . . . . . . . "Llibres electrònics" . . . . . . . "Aufsatzsammlung" . . . "Electronic books"@en . . . . . . . . . . . . "Elektronisches Buch" . . . . "In vitro mutagenesis protocols" . "In vitro mutagenesis protocols"@en . "Gidsen (vorm)" . . . . . "In the post-genomic era, in vitro mutagenesis has emerged as a critically important tool for establishing the functions of components of the proteome. The third edition of In Vitro Mutagenesis Protocols represents a practical toolbox containing protocols vital to advancing our understanding of the connection between nucleotide sequence and sequence function. Fully updated from the previous editions, this volume contains a variety of specialty tools successfully employed to unravel the intricacies of protein-protein interaction, protein structure-function, protein regulation of biological processes, and protein activity, as well as a novel section on mutagenesis methods for unique microbes as a guide to the generalization of mutagenesis strategies for a host of microbial systems. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, In Vitro Mutagenesis Protocols, Third Edition offers today's researchers a valuable compendium of reliable and powerful techniques with which to illuminate the proteome and its rich web of biological implications."@en . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . "Metoder" . . "Mutagenèse Méthodologie." . . "Cell Biology." . . "DNA" . . . .