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Vaccine Protocols

This extensively revised, updated, and expanded second edition of a classic laboratory manual applies new understanding of molecular mechanisms to the rational design and development of vaccines. Here the working researcher will find readily reproducible techniques for the development and production of live attenuated vaccines through the genetic manipulation of viruses and bacteria, vaccine vectors or inactivated toxins, and the production of synthetic peptides and conjugate vaccines. In addition, there are chapters describing more general techniques for vaccine formulation, delivery, and assessment of immune responses, and review discussions of scale-up to manufacture, vaccine quality assurance, and clinical trials. New to this second edition are cutting-edge chapters on two different viral vectors (Vaccinia and Semliki Forest), lactic acid bacteria for delivery of foreign antigens, incorporation of immunomodulators into plasmid DNA vaccines, construction of tetramer complexes to detect CMI responses, assessment of functional antibody responses, and the use of complete genome sequences for vaccine design. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results. State-of-the-art and highly practical, Vaccine Protocols, Second Edition, provides essential information for those seeking to produce improved or novel vaccines in the rapidly evolving field of vaccine research and development.

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  • "This extensively revised, updated, and expanded second edition of a classic laboratory manual applies new understanding of molecular mechanisms to the rational design and development of vaccines. Here the working researcher will find readily reproducible techniques for the development and production of live attenuated vaccines through the genetic manipulation of viruses and bacteria, vaccine vectors or inactivated toxins, and the production of synthetic peptides and conjugate vaccines. In addition, there are chapters describing more general techniques for vaccine formulation, delivery, and assessment of immune responses, and review discussions of scale-up to manufacture, vaccine quality assurance, and clinical trials. New to this second edition are cutting-edge chapters on two different viral vectors (Vaccinia and Semliki Forest), lactic acid bacteria for delivery of foreign antigens, incorporation of immunomodulators into plasmid DNA vaccines, construction of tetramer complexes to detect CMI responses, assessment of functional antibody responses, and the use of complete genome sequences for vaccine design. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results. State-of-the-art and highly practical, Vaccine Protocols, Second Edition, provides essential information for those seeking to produce improved or novel vaccines in the rapidly evolving field of vaccine research and development."@en
  • "Expert practitioners present a comprehensive approach to vaccine development, production, and assessment that can be adapted successfully to any vaccine. Their approach not only includes state-of-the-art techniques for developing live viral and bacterial vectors, expressing foreign vaccine antigens, and producing peptide and conjugate vaccines, but also offers advice and guidance on clinical trials and regulatory requirements. Additional techniques describe such powerful tools as the use of adjuvants, freeze drying vaccines, microencapsulation of vaccines, the full assessment of immunogenicity and potency of vaccines in animal models, and the creation of DNA vaccines, chimeric proteins, and recombinant toxoids."@en
  • "Expert practitioners present a comprehensive approach to vaccine development, production, and assessment that can be adapted successfully to any vaccine. Recent advances in vaccine research include those in the areas of bacterial genomics, antigen-specific T Cell quantification, genetic manipulation of vaccine vectors, the harnessing of natural molecules concerned with the regulation of immune responses, and the burgeoning field of DNA vaccinology. This second edition has been thoroughly revised with new chapters on live viral vaccine vectors, attenuated bacterial vectors, immunomodulators, MHC-peptide tetrameric complexes and the identification of vaccine candidates by genomic analysis. Additionally, chapters from the first edition have been updated to accommodate state-of-the-art methods in molecular vaccinology, making this a valuable and relevant practical manual for all those seeking to produce improved or novel vaccines."

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  • "Online-Publikation"
  • "Handbooks, manuals, etc"@en
  • "Gidsen (vorm)"
  • "Elektronisches Buch"
  • "Handbooks, manuals, etc"
  • "Electronic books"@en
  • "Handbooks and manuals"
  • "Llibres electrònics"

name

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  • "Vaccine Protocols"@en
  • "Vaccine Protocols"
  • "Vaccine protocols"@en
  • "Vaccine protocols"

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