"Cartographie d'interactions entre protéines." . . "Interactions protéine-protéine Manuels de laboratoire." . . "Interactions protéine-protéine - Manuels de laboratoire." . . . . . . . . . "Llibres electrònics" . . . . . . . . . . . . "Handbooks, manuals, etc"@en . "Handbooks, manuals, etc" . . . . . . . . . . . . . . . . . "Protein-protein interactions : Methods and applications" . . "Laboratory manuals"@en . . "Laboratory manuals" . . . . . . . . "In recent years, our understanding of the protein-protein interaction landscape has expanded significantly. Growing interest in analyzing and targeting protein-protein interactions has resulted in an increased need for detailed methodologies to interrogate and monitor protein-protein interactions. To meet this need, Protein-Protein Interactions: Methods and Applications has been updated and expanded. The second edition includes core technological platforms used to study protein-protein interactions, and cutting-edge technologies that reflect recent scientific advances and the emerging focus on therapeutic discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of necessary materials and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. These well-detailed protocols describe methods for identifying protein-protein interaction partners, analyzing protein-protein interactions quantitatively and qualitatively, monitoring protein-protein interactions in live cells, and predicting and determining interaction interfaces. Authoritative and cutting-edge, Protein-Protein Interactions: Methods and Applications, Second Edition is a valuable resource that will enable readers to elucidate the mechanisms of protein-protein interactions, determine the role of these interactions in diverse biological processes, and target protein-protein interactions for therapeutic discovery.{OCLCbr#A0}."@en . . "Electronic resource"@en . . . . "Protein-Protein Interactions : Methods and Applications" . . . . . . . . "A collection of highly successful biochemical, biophysical, genetic, and computational techniques for studying protein-protein interactions. These readily reproducible methods demonstrate how to identify protein interaction partners, qualitatively or quantitatively measure protein-protein interactions in vitro or in vivo, monitor protein-protein interactions as they occur in living cells, and determine interaction interfaces. The techniques described utilize a variety of cutting-edge technologies, including surface plasmon resonance (SRP), fluorescence resonance energy transfer (FRET), fluorescence polarization (FP), isothermal titration calorimetry (ITC), circular dichroism (CD), protein fragment complementation assays (PCA), various two-hybrid systems, and proteomics and bioinformatics-based approaches, such as the Scansite program for computational analysis. These powerful tools are essential for deciphering how proteins interact with each other to form biological networks, as well as for unraveling protein-protein interactions in disease to find novel therapeutic targets."@en . . . "Protein-Protein Interactions Methods and Applications" . "Protein-Protein Interactions Methods and Applications"@en . . . . . . . . "Protein-protein interactions : methods and applications"@en . . . "Protein-protein interactions : methods and applications" . "Aufsatzsammlung" . . "Proteins continuously interact with each other to determine cell fate. Consequently, an examination of just when such protein-protein interactions occur and how they are controlled is essential for understanding the molecular mechanism of biological processes, elucidating the molecular basis of diseases, and identifying potential targets for therapeutic interventions. In Protein-Protein Interactions: Methods and Applications, leading experts describe in detail their highly successful biochemical, biophysical, genetic, and computational techniques for studying these interactions. Their readily reproducible methods demonstrate how to identify protein interaction partners, qualitatively or quantitatively measure protein-protein interactions, monitor protein-protein interactions as they occur in living cells, and determine interaction interfaces. The techniques described utilize a variety of cutting-edge technologies, including surface plasmon resonance (SRP), fluorescence resonance energy transfer (FRET), fluorescence polarization (FP), isothermal titration calorimetry (ITC), circular dichroism (CD), protein fragment complementation assays (PCA), various two-hybrid systems, and proteomics- and bioinformatics-based approaches, such as the Scansite program for computational analysis. Each time-tested protocol includes a background introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and highly practical, Protein-Protein Interactions: Methods and Applications offers both beginning and experienced investigators a full range of the powerful tools needed for deciphering how proteins interact to form biological networks, as well as for unraveling protein-protein interactions in disease in the search for novel therapeutic targets."@en . . "Protein-protein interactions (Fu)" . "Protein-protein interactions methods and applications" . . "Online-Publikation" . "Protein-protein interactions methods and applications"@en . . . . . . . . . . . . . "Laboratory Manuals" . . . "In recent years, our understanding of the protein-protein interaction landscape has expanded significantly. Growing interest in analyzing and targeting protein-protein interactions has resulted in an increased need for detailed methodologies to interrogate and monitor protein-protein interactions. To meet this need, Protein-Protein Interactions: Methods and Applications has been updated and expanded. The second edition includes core technological platforms used to study protein-protein interactions, and cutting-edge technologies that reflect recent scientific advances and the emerging focus on therapeutic discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of necessary materials and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. These well-detailed protocols describe methods for identifying protein-protein interaction partners, analyzing protein-protein interactions quantitatively and qualitatively, monitoring protein-protein interactions in live cells, and predicting and determining interaction interfaces. Authoritative and cutting-edge, Protein-Protein Interactions: Methods and Applications, Second Edition is a valuable resource that will enable readers to elucidate the mechanisms of protein-protein interactions, determine the role of these interactions in diverse biological processes, and target protein-protein interactions for therapeutic discovery." . . . "Electronic books"@en . . "Drug Discovery Laboratory manuals." . . "SCIENCE Life Sciences Biochemistry." . . "Analyser" . . "Analysis" . . "Protein-protein interactions." . . "Reação em cadeia por polimerase." . . "Kromatografi" . . "Kalorimetri" . . "Protein-Protein-Wechselwirkung." . . "Protein Science." . . "Protein Interaction Mapping Laboratory manuals." . . "Ressonância magnética nuclear." . . "Bioquímica" . . "Biochemistry." . . "Proteins" . . "Chromatography" . . "Spektrometri, spektroskopi" . . "Proteïnes Metabolisme." . . "Protéines métabolisme." . "Drug development Laboratory manuals." . . "Metoder" . . "Proteínas (metabolismo)" . . "Manual" . . "Proteins metabolism Laboratory Manuals." . . "Proteins metabolism Laboratory Manuals" . "Biowissenschaften." . . . . "Protéines." . . "Mikroskopi" . . "Electronic books." . . "interaction intermoléculaire protéine technique de laboratoire [manuel]" . . "Microscopy" . . "Ciències de la vida" . . "Electrophoresis" . . "Proteiner" . . "Protein-protein interactions Laboratory manuals." . . "Life sciences." . . "Life Sciences" . "Drug development." . . "Liaison aux protéines physiologie." . . "Methods" . . "Biochemistry, general" . . "Biologia molecular (métodos;aplicações)" . . "Elektroforese" . . "Calorimetry" . . "Protein Binding physiology Laboratory Manuals." . . "Protein Binding physiology Laboratory Manuals" . "Labortechnik." . . "Genètica." . . "Vejledning" . . "Protein Binding Laboratory manuals." . . "Mutação genética." . . "Interactions protéine-protéine." . . "Spectrometry" . .