"Genetic engineering" . . "Biochemistry, general" . . "Human diseases" . . "Proteínas." . . "Anticossos." . . "Rekombination" . . "Internet" . . "Gentechnisch erzeugtes Produkt." . . "Bioquímica" . . "Genetics" . . "Kloning" . . "Antibodies Laboratory Manuals therapeutic use." . . "Genetik" . . "Methods" . . "Monoklonaler Antikörper." . . . . . . . . . "Antibody engineering : methods and protocols" . "Antibody Engineering Methods and Protocols" . . . . . . . . . . "Electronic books" . "Electronic books"@en . . . . "Antibody Engineering: Methods and Protocols" . . . . . . . . . . . . . . "With 10 recent FDA approvals of therapeutic antibody drugs and hundreds more in the biotechnology pipeline, it is clear that engineered antibodies will play a major role in treating human diseases in the 21st century. In Antibody Engineering: Methods and Protocols, leading researchers and clinicians present a core collection of cutting-edge techniques for the generation, expression, optimization, and characterization of recombinant antibodies. Readily reproducible protocols for lead generation range from the cloning of human immunoglobulin genes to the generation of human recombinant antibodies by humanization approaches, molecular display technologies, and transgenic animals. The techniques for antibody expression and optimization utilize not only bacterial and mammalian cell cultures, but also insect cell cultures and transgenic plants. State-of-the-art technologies are described for the characterization of antigen-binding affinity and specificity with novel applications in radioimmunotargeting, cancer immunotherapy, drug abuse, and proteomics. Each fully tested protocol is described in step-by-step detail and includes background material, equipment and reagent lists, and tips on troubleshooting and avoiding pitfalls. Comprehensive and up-to-date, Antibody Engineering: Methods and Protocols provides both laboratory and clinical investigators with a diverse array of the fundamental techniques upon which new and imaginative drug technologies can be successfully developed."@en . "Handbooks and manuals" . . . . . . . . . "Llibres electrònics" . . . . . . . "Handbooks, manuals, etc"@en . . . . . "Handbooks, manuals, etc" . . . . . . . . . . . . . . . "Online-Publikation" . . . . . . . . . . . . . . . . . . "Antibody engineering methods and protocols" . . "Antibody engineering methods and protocols"@en . "Aufsatzsammlung" . . . . . . "MEDICAL Immunology." . . "Immunotecnologia." . . "Antibodies genetics Laboratory Manuals." . . "Antibodies genetics Laboratory Manuals" . "Chromatography" . . "Purification" . . "Ingénierie de protéines." . . "Databases" . . "Antibodies therapeutic use Laboratory Manuals." . . "Antibodies therapeutic use Laboratory Manuals" . "Bioinformatics" . . "Cloning" . . "Aufsatzsammlung." . . "Arzneimittelentwicklung." . . "Antibodies" . . "Protein Engineering Laboratory Manuals methods." . . "Pharmazeutische Technologie." . . "Kromatografi" . . "Metoder" . . "Immunologie Technologie." . . "Enginyeria de proteïnes." . . "Databaser" . . "Recombination" . . . . "Antistoffen." . . "Life Sciences" . . "Protein Engineering methods Laboratory Manuals." . . "Protein Engineering methods Laboratory Manuals" . "Anticorps Emploi en thérapeutique." . . "Biowissenschaften." . . "Immunotechnology." . . "Pharmazeutische Technologie Monoklonaler Antikörper Aufsatzsammlung." . . "Bioinformatik" . . "Imunologia." . . "Arzneimittelentwicklung Gentechnisch erzeugtes Produkt Monoklonaler Antikörper Aufsatzsammlung." . . "Genetische manipulatie." . . "Vejledning" . . "Genteknologi" . . "Antibodies Laboratory Manuals genetics." . . "Anticorpos (genética)" . . "Anticorpos (genética)." . "Wetenschappelijke technieken." . . "Antistoffer" . . "Sygdomme mennesker" . . "Raffinering, oprensning" . . "Lo, Benny K. C." . . "Manual" . . "Ciències de la vida" . . "Immunoglobulius Biotechnology" . . "Electronic books." . . "Immunotechnologie Manuels de laboratoire." . . "Terapêutica." . .