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Immunoblotting

The video explains the principles of immunoblotting with the aid of diagrams and demonstrates the technique in the laboratory. Immunoblotting explains why attempting to detect the presence of protein samples directly from an electrophoresis gel is impractical, due to diffusion of the protein bands over time and the fragility of the gel itself. The principles of immunoblotting are demonstrated and the transferring of information from the gel to a nitrocellulose membrane is described with the aid of diagrams. The techniques are then demonstrated in the laboratory by showing a method of making a single specific protein on the membrane visible using an antibody to that protein. The adding of a second antibody with an enzyme attached that will produce a coloured product when a substrate is added, is also unknown.

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  • "The video explains the principles of immunoblotting with the aid of diagrams and demonstrates the technique in the laboratory. Immunoblotting explains why attempting to detect the presence of protein samples directly from an electrophoresis gel is impractical, due to diffusion of the protein bands over time and the fragility of the gel itself. The principles of immunoblotting are demonstrated and the transferring of information from the gel to a nitrocellulose membrane is described with the aid of diagrams. The techniques are then demonstrated in the laboratory by showing a method of making a single specific protein on the membrane visible using an antibody to that protein. The adding of a second antibody with an enzyme attached that will produce a coloured product when a substrate is added, is also unknown."@en
  • "(Producer) This program explains the principles of immunoblotting with the aid of computer diagrams and demonstrates how to implement the technique in the laboratory. It explains why attempting to detect the presence of protein samples directly from an electrophoresis gel is impractical, due to diffusion of the protein bands over time and the fragility of the gel itself. The technique of immunoblotting is then illustrated in the laboratory, by showing a method of making a single specific protein visible by using an antibody to that protein. The effect of adding a second antibody with an enzyme attached to produce a colored product when a substrate is added is also shown."
  • "Part of a four-part series showing how to properly take samples and incorporate the specific techniques needed to complete laboratory tests and carry out research. This segment explains the principles of immunoblotting with the aid of computer diagrams and demonstrates how to implement the technique in the laboratory. Explains why attempting to detect the presence of protein samples directly from an electrophoresis gel is impractical, due to diffusion of the protein bands over time and the fragility of the gel itself. The technique of immunoblotting is then illustrated in the laboratory, by showing a method of making a single specific protein visible by using an antibody to that protein. The effect of adding a second antibody with an enzyme attached to produce a colored product when a substrate is added is also shown."
  • "This DVD explains the principles of immunoblotting with the aid of diagrams and demonstrates the technique in the laboratory. It also explains why attempting to detect the presence of protein samples directly from an electrophoresis gel is impractical, due to diffusion of the protein bands over time and the fragility of the gel itself."@en
  • "Explains the principles of immunoblotting with the aid of computer diagrams and demonstrates how to implement the technique in the laboratory. Discusses why attempting to detect the presence of protein samples directly from an electrophoresis gel is impractical, due to diffusion of the protein bands over time and the fragility of the gel itself. Illustrates the technique of immunoblotting by showing a method of making a single specific protein visible by using an antibody to that protein. Shows the effect of adding a second antibody with an enzyme attached to produce a colored product when a substrate is added."
  • "(Producer) This program explains the principles of immunoblotting with the aid of computer diagrams and demonstrates how to implement the technique in the laboratory. It explains why attempting to detect the presence of protein samples directly from an electrophoresis gel is impractical, due to diffusion of the protein bands over time and the fragility of the gel itself. The technique of immunoblotting is then illustrated in the laboratory, by showing a method of making a single specific protein visible by using an antibody to that protein. The effect of adding a second antibody with an enzyme attached to produce a colored product when a substrate is added is also shown."
  • "The principles of immunoblotting are demonstrated and the transferring of information from the gel to a nitrocellulose membrane is described with the aid of diagrams."

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  • "Immunoblotting"
  • "Immunoblotting"@en

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