"Basic Methods in Molecular Biology discusses the heart of the most recent revolution in biology-the development of the technology of genetics. The achievements in this field have simply changed what biologists do and, perhaps even more important, the way they think. Moreover, never before have scientists from such a broad range of disciplines rushed into such a small and slightly arcane field to learn and carry off a bit of the technology. This book comprises 21 chapters, opening with three introductory ones that discuss the basics of molecular biology; the tools of the molecular biologist; an..."
"Basic Methods in Molecular Biology."@en
"1: The basics of molecular biology. 2: The tools of the molecular biologist. General preparations, procedures, and considerations for use of manual. 4: Cloning vectors and bacterial cells. Preparation of DNA form eukaryotic cells. 6: Probing nucleic acids with labeled synthetic probes. 7: Probing nucleic acids with plasmid-derived probes. 8: Plasmid DNA preparation. 9: DNA Restriction fragment preparation. 10: Purification of DNA. 11: Preparation and analysis of RNA from eukaryotic cells. 12: Preparation of DNA from bacteriophage clones. Cloning DNA from the eukaryotic genome. 14: cDNA cloning into gt10 and gt11. 15: Subcloning into plasmids. 16: M13 cloning and sequencing. 17: Further characterization of cloned DNA. 18: Transfection of mammalian cells in culture. 19: Protein methods. 20: General methods. Specialized methods."