http://worldcat.org/entity/work/id/7658947

Ultracytochemistry of intracellular membrane glycoconjugates

This investigation is concerned with the ultracytochemistry of glycoconjugates - i. e. , the carbohydrate moieties of glycoproteins and glycolipids - attached to intracellular post-Golgi membranes (membranes of lysosomes, peroxisomes, secretory granules of exocrine and endocrine gland cells). In addition mitochon dria have been studied. There are at present very few cytochemical studies, none of them systematic, on intracellular membrane-bound glycoconjugates. So far it has only been re ported that phosphotungstic acid (PTA) at low pH (pH < 0. 5) produces a rim of electron-dense material on the inner surface of the lysosomal membrane in lysosomes of liver, kidney, adenohypophysis, and of macrophages from the spleen in rats and mice (Daems and Persijn 1964; Rambourg 1967; Rambourg and Racadot 1968; Babai and Bernhard 1971; see also literature cited in Daems et al. 1969, 1972), in liver lysosomes in the brook-lamprey (Lampetra zanandreai; Bertolini 1965) and in lysosomes of male accessory glands in Drosophila melano gaster (Perrin-Waldemer 1977). Besides this, with the same method an identical ring-like staining has been detected in secretory granules of the adenohypophysis (Rambourg and Racadot 1968; Tixier-Vidal and Picart 1971; Pelletier 1971; Warchol et al. 1976) and in renin granules (Cantin et al. 1975). However, the cytochemical specificity of PTA at low pH has been at best incompletely estab lished in these studies.

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