Cellules eucaryotes Manuels de laboratoire.
Microbiologie moleculară Manual universitar Lucrări practice.
Clonación molecular Manuales de laboratorio.
Celule eucariote Manual universitar Lucrări practice.
Clonazione molecolare - Manuali per laboratori.
Células eucarióticas Manuales de laboratorio.
Clonage moléculaire Manuels de laboratoire.
Genetica Molecular E De Microorganismos.
Molecular cloning : A laboratory manual 1-3
Plasmid vectors; Bacteriophage vectores; Cosmid vectors; Single-stranded; Filamentous bacteriophage vectors; Enzymes used in molecular cloning; Gel electrophoresis of DNA; Extraction, Purification, and anlysis of messenger RNA from eukaryotic cells;
V.1: Plasmids and their usefulness in molecular cloning; Bacteriophage and its vectors; Working with bacteriophage M13 vectors; Working with high-capacity vectors; Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis; Preparation and analysis of eukaryotic genomic DNA; Extraction, purification, and analysis of mRNA from eukaryotic cells; v.2: In vitro amplification of DNA by the polymerase chain reaction; Preparation of radiolabeled DNA and RNA probes; Working with synthetic oligonucleotide probes; Preparation of cDNA libraries and gene identification; DNA sequencing; Mutagenesis; Screening expression libraries; v.3: Expression of cloned genes in escherichia coli; Introducing cloned genes into cultured mammalian cells; Analysis of gene expression in cultured mammalian cells; Protein interaction technologies.
Molecular cloning
Molecular cloning a laboratory manual
Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential. ... The theoretical and historical underpinnings of techniques are prominent features of the presentation throughout, information that does much to help trouble-shoot experimental problems. For the fourth edition of this classic work, the content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories. Core chapters from the third edition have been revised to feature current strategies and approaches to the preparation and cloning of nucleic acids, gene transfer, and expression analysis. They are augmented by 12 new chapters which show how DNA, RNA, and proteins should be prepared, evaluated, and manipulated, and how data generation and analysis can be handled. The new content includes methods for studying interactions between cellular components, such as microarrays, next-generation sequencing technologies, RNA interference, and epigenetic analysis using DNA methylation techniques and chromatin immunoprecipitation. To make sense of the wealth of data produced by these techniques, a bioinformatics chapter describes the use of analytical tools for comparing sequences of genes and proteins and identifying common expression patterns among sets of genes. Building on thirty years of trust, reliability, and authority, the fourth edition of Molecular Cloning is the new gold standard--the one indispensable molecular biology laboratory manual and reference source. --Publisher description.
Molecular cloning : a laboratory manual
Molecular cloning / Tom Maniatis, Ed F. Fritsch, Joe Sambrook
V. 1: Plasmids and their usefulness in molecular cloning. Bacteriophage and its vectors. Working with bacteriophage M13 vectors. Working with high-capacity vectors. Gel electrophoresis of DNA and pulsed-field agarose gel electriphoseris. Preparation and analysis of eukaryotic genomic DNA. Extraction purification and analysis pf mRNA from eukaryotic cells. V.2: In vitro amplification of DNA by the polymerase chain reaction. Preparation of radialabeled DNA and RNA probes. Working wih synthetic oligonucleotide probles. Preparation of cDNA libraries and gene identification. DNA sequencing. Mutagenesis. Screening expression libraries. Expression of cloned genes in Escherichia coli. V. 3: Induncing cloned genes into cultured mammalian cells. Analysis of gene expression in cultured mammalian cells. Protein interaction technologies.
Handbooks, manuals, etc
Clonare Manual universitar Lucrări practice.
Cèl·lules eucariotes Manuals de laboratori.
Clonació molecular Manuals de laboratori.
Clonamiento molecular Manuales de laboratorio.
Clonación molecular Manual de laboratorio.
Biologia molecolare - Manuali per laboratori.
Klonowanie molekularne podręczniki laboratoryjne.
Biológia molekuláris laboratóriumi technika.
Genetica molecular Manuales de laboratorio.
Clonatge molecular Manuals de laboratori.