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Photosynthesis Research Protocols

The capture of sunlight by photosynthetic organisms supplies an enormous amount of the energy required to develop and sustain life on the planet, making photosynthesis one of the most important biological phenomenon on Earth. Updating the popular first edition, Photosynthesis Research Protocols, Second Edition presents detailed descriptions of a broad range of general and fundamental methods that are commonly used by plant biochemists, physiologists, and molecular biologists, all of which are contributed by leading researchers in the field. This vital new edition also features coverage of methods related to the most abundant protein on earth, Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase. Written in the highly successful Methods in Molecular Biology series format, the chapters of this volume include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Photosynthesis Research Protocols, Second Edition serves new photosynthesis researchers as well as experienced scientists seeking to use a new type of preparation or method to study this tremendously influential process.

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  • "Fractionations of Thylakoid membranes into grana and stroma thylakoids; Isolation of photosystem I particles from spinach; Rapid isolation and purification of photosystem I chlorophyll-binding protein from Chlamydomonas reinhardtii; Isolation of photosystem II-enriched membranes and the oxygen-evolving complex subunit proteins from higher plants; Isolation of functional photosystem II core particles from the cyanobacterium synechocystis sp. PCC 6803; Isolation of photosystem I reaction center preparation from spinach; Isolation of photosystem II reaction center complexes from plants; Isolation of photosystem I reaxtion center subunit polypeptides form spinach; Purificatoin and crystallizaton of the cytochrome b6f complex in oxygenic photosynthesis; Purification of plastocyanini and cytochorme c6 from plants green algae, and cyanobacteria; Preparation fo native recombinant light-harvesting chlorophyll-a/b complex; Isolation and characterization of lamellar aggregates of LHCII structural transitions; Separation, purification, and characterization of polypeptide composition of subcomplexes of the main light-harvesting chlorophyll a/b-protein complex of photosystem II; Isolation of CP43 and CP47 photosystem II proximal antenna complexes from plants; Determination and quantification of photosynthetic pigments by reserve phase high-performance liquid chromatography, thin-layer chromatography, and spectrophotometry; Isolation and identificaton of chloroplast lipids; DNA adducts with chlorophyll and chlorophyllin as antimutagenic agents: synthesis, stability, and structural features; Incorporation and analysis of LHCII in model systems; Photosystem II reconstitution into proteoliposomes: structure-function characterization; Extraction of the functional manganese and calcium from photosystem II; Assay of photoinhibition of photosystem II and protease activity..."
  • "The capture of sunlight by photosynthetic organisms supplies an enormous amount of the energy required to develop and sustain life on the planet, making photosynthesis one of the most important biological phenomenon on Earth. Updating the popular first edition, Photosynthesis Research Protocols, Second Edition presents detailed descriptions of a broad range of general and fundamental methods that are commonly used by plant biochemists, physiologists, and molecular biologists, all of which are contributed by leading researchers in the field. This vital new edition also features coverage of methods related to the most abundant protein on earth, Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase. Written in the highly successful Methods in Molecular Biology series format, the chapters of this volume include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Photosynthesis Research Protocols, Second Edition serves new photosynthesis researchers as well as experienced scientists seeking to use a new type of preparation or method to study this tremendously influential process."@en

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  • "Aufsatzsammlung"
  • "Llibres electrònics"
  • "Handbooks, manuals, etc"@en
  • "Handbooks, manuals, etc"
  • "Online-Publikation"
  • "Electronic books"@en

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  • "Photosynthesis Research Protocols"
  • "Photosynthesis Research Protocols"@en
  • "Photosynthesis research protocols"@en
  • "Photosynthesis research protocols"

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