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Manipulation and expression of recombinant DNA : a laboratory manual

Introduces advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein.

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  • "(Publisher-supplied data) This laboratory manual will be an indispensable tool for students, by providing in depth descriptions of protocols which cover the basic aspects of molecular biology and biochemistry. The book covers techniques in detail, and teaches students to culture and transform bacteria, grow vector plasmids, purify insert DNA, ligate vector and insert, and ultimately express protein and assay its activity. Comprehensive and complete, this text covers the underlying techniques used in all basic research and biotechnology laboratories. Key Features Designed to cover the basic aspects of molecular biology and biochemistry. Features student-tested protocols. Presents experiments in a modular fashion, lending itself equally to the traditional semester course and the shorter intensive course. Covers important techniques and protocols used in all basic research labs. "Project" approach to experiments gives students an overview of entire process. Experiments incorporate immunological techniques that go beyond basic exercises in DNA cloning."
  • "Introduces advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein."@en
  • "This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein. Students can actually visualize positive clones following IPTG induction."

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  • "Praktikum"
  • "Electronic books"
  • "Electronic books"@en
  • "Livres électroniques"
  • "Libros electrónicos"
  • "Handbooks, manuals, etc"
  • "Handbooks, manuals, etc"@en

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  • "Manipulation and Expression of Recombinant DNA : a laboratory manual"
  • "Manipulation and expression of recombinant DNA : a laboratory manual"@en
  • "Manipulation and expression of recombinant DNA : a laboratory manual"
  • "Manipulation and expression of recombinant DNA a laboratory manual"
  • "Manipulation and expression of recombinant DNA a laboratory manual"@en
  • "Manipulation and expression of recombinant DNA"
  • "Manipulation and Expression of Recombinant DNA"

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