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http://worldcat.org/entity/work/id/487962289

An Epidermal Biosensor for Carcinoembryonic Antigen

The goal of this grant is to develop a prototype epidermal biosensor for carcinoembryonic antigen (CEA) as an early, sensitive detector of the onset of breast cancer. An epidermal biosensor represents a new method of disease detection in which a small area of skin containing modified keratinocytes recognizes and responds to molecules secreted into the circulation by a tumor. In the first year of the grant, epidermal keratinocytes were genetically modified with a retroviral vector to express a chimeric cell surface receptor that recognizes CEA. The transduced cells were shown to bind CEA, unlike untransduced, normal keratinocytes. Using this information, chimeric receptors with an intracellular domain from tumor necrosis factor a receptor I are being designed so that CEA binding will bring about a cellular response in vifro and initiate a local inflammatory reaction in vivo. The long-term objective is to explore the use of epidermal biosensors as a continuous, in vivo monitors for the presence of tumor antigens such as CEA. The expectation is that epidermal biosensors will provide early detection of the onset of disease in high-risk patients so that appropriate medical management could & initiated when it is most likely to result in a positive outcome.

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  • "The goal of this grant is to develop a prototype epidermal biosensor for carcinoembryonic antigen (CEA) as an early, sensitive detector of the onset of breast cancer. An epidermal biosensor represents a new method of disease detection in which a small area of skin containing modified keratinocytes recognizes and responds to molecules secreted into the circulation by a tumor. In the first year of the grant, epidermal keratinocytes were genetically modified with a retroviral vector to express a chimeric cell surface receptor that recognizes CEA. The transduced cells were shown to bind CEA, unlike untransduced, normal keratinocytes. Using this information, chimeric receptors with an intracellular domain from tumor necrosis factor a receptor I are being designed so that CEA binding will bring about a cellular response in vifro and initiate a local inflammatory reaction in vivo. The long-term objective is to explore the use of epidermal biosensors as a continuous, in vivo monitors for the presence of tumor antigens such as CEA. The expectation is that epidermal biosensors will provide early detection of the onset of disease in high-risk patients so that appropriate medical management could & initiated when it is most likely to result in a positive outcome."@en
  • "The goal of this grant was to develop a prototype epidermal biosensor for carcinoembryonic antigen (CEA) An epidermal biosensor was conceived as a new approach for the early continuous, in vivo detection of the onset of disease by the using genetically modified skin cells to respond to molecules secreted by tumor cells. The research we have conducted has allowed us to conclude that human keratinocytes in vitro can be engineered to express a chimeric cell surface receptor and that these modified cells could recognize and bind CEA Technical problems in generating chimeric receptor constructs were resolved but we were unable to create a chimeric cell surface receptors that had domains that would bind and respond to CEA in human keratinocytes In the absence of supplemental finding, we were unable to pursue other avenues for constructing cell surface receptors that would introduce into keratinocytes the capacity to act as in vivo biosensors. We remain confident that such chimeric receptors can be generated to respond to tumor antigens such as CEA. We believe that keratinocyte biosensors could be effective early detection systems and play an important role in the management of breast cancer."@en

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  • "An Epidermal Biosensor for Carcinoembryonic Antigen"@en

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